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Screen any human variant in zebrafish

Day 1 Dosing

Day 3 Screening

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When to use zebrafish

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Strengthen FDA Submissions: Build robust evidence

Streamline Lead Optimization: Select compounds confidently   
Accelerate Mechanism Discovery: Uncover biological pathways

Deliver Proof-of-Concept: Validate data visually                        

How to dose the fish?
 

Water dissolution carries DMSO soluble drugs directly into the blood stream through the skin. Other dosing routes are available too.

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 How long does it take to see results?

1 week: ECG, EEG,  Sleep, Metastasis, Hair cells function

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2 weeks: Locomotion, Balance, Fear, Learning, Auditory, Visual test, Protein degradation

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3 weeks:  Biochemistry, ALT, AST, CSF Biomarkers, Obesity, MASH, Aging, Fibrosis

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4 weeks:  Histology and Immunohistochemistry, Diet induced, Cardiovascular, Arthritis

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5 weeks: Carcinogenicity

How to apply the model in Early Discovery Studies?

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Efficacy Screening – Identify potency compounds, rescue genetic variants

 

Target Validation

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Biomarker rescue

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Molecular Mechanism Validation

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Clinical Endpoint Scoring  - HOMA IR, MASH Score, Epilepsy Score

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"We Specialize in Measuring Human Endpoints in Zebrafish"

Adherence to the highest standards of animal care

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Current and Active 

Leadership

Scientific Advisory Board

Key Members

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 DELIVERING EXCEPTIONAL WORLDWIDE SERVICES

Pentagrit In Numbers

140+

Annual Studies Globally

60

Days to Study Turn Around

90%

Mutant coverage of Human Targets

All

Clinical Assays done in Zebrafish

Publications

  1. Joseph B, Kalaichitra P, Kar B, Vishnupriya D, Muthulakshmi V. Method development and validation of short-chain brain sulfatide using RP-HPLC UV. Asian Journal of Pharmaceutical Research and Development. 2026;14(1):11-14.

  2. Acton S, O’Donnell MM, Kalaichitra P, Dixit B, Eishingdrelo H, Hill C, Ross RP, Chesnel L. LPA3 agonist-producing Bacillus velezensis ADS024 is efficacious in multiple neuroinflammatory disease models. Brain, Behavior, and Immunity. 2024;121:384-402.

  3. Acton S, Meilin S, Schulman Y, Aran J, Kalaichitra P, and Chesnel L ADS024, a single strain live biotherapeutic product, attenuates MOG-EAE induced clinical symptoms in mice and zebrafish. Multiple Sclerosis Journal. 2023; Volume 29, Issue 3_suppl:P1459/415 ePoster.

  4. Fazeela MBSM, Kalaichitra P, Joseph B, Sundararajan R, Hemalatha S. Gelidiella acerosa inhibits lung cancer proliferation. BMC Complementary and Alternative Medicine. 2018;18(1):104-118.

  5. Sivamani S, Joseph B, Kar B. Zebrafish: an emerging model system for drug discovery. Asian Journal of Pharmaceutical and Clinical Research. 2016;9(4):11-14.

  6. Sivamani S, Joseph B, Kar B. Anti-inflammatory activity of Withania somnifera leaf extract in stainless steel implant induced inflammation in adult zebrafish. Journal of Genetic Engineering and Biotechnology. 2014;12(1):1-6.

  7. Sivamani S, Joseph B, Nishwetha K, Kar B. Zebrafish as a model for bioavailability testing of over the counter drug. International Journal of Drug Development and Research. 2013;5:159-163.

  8. Sivamani S, Kar B. Adult zebrafish as a new animal model to study anxiety. Asian Journal of Experimental Biological Science. 2013;4:167-171.

  9. Sivamani S, Joseph B, Kar B. Zebrafish: an in vivo model for the study of liposome mediated drug delivery. International Journal of Molecular Genetics. 2013;3:53-61.

  10. Kar B, Sivamani S. Zebrafish: an in vivo model for the study of human diseases. International Journal of Genetics and Genomics. 2013;1(1):6-11.

Associated Publications

  1. Prakash SK. Immunogenic antitumor potential of Prakasine nanoparticles in zebrafish by gene expression stimulation. Artificial Cells, Nanomedicine, and Biotechnology. 2023;51(1):41-56.

  2. Balkrishna A, Nain P, Joshi M, Kumar B, Varshney A. Super-critical fluid extract of Bryonopsis laciniosa (Shivlingi) seeds restores fertility in zebrafish models through revival of cytological and anatomical features. Journal of Ovarian Research. 2022;15(1):46-65.

  3. Balkrishna A, Lochab S, Joshi M, Srivastava J, Varshney A. Divya-herbal-peya decoction harmonizes the inflammatory response in lipopolysaccharide-induced zebrafish model. Journal of Experimental Pharmacology. 2021;13:937-955.

  4. Vinola SMJ, Karthikeyan K, Sharma A, Sudheshna S, Sekar M. Anti-inflammatory efficacy of petasin-incorporated zinc oxide eugenol sealer–An in vivo zebrafish study. Journal of Conservative Dentistry. 2021;24(6):539-543.

  5. Balkrishna A, Verma S, Solleti SK, Khandrika L, Varshney A. Calcio-herbal medicine Divya-Swasari-Vati ameliorates SARS-CoV-2 spike protein-induced pathological features and inflammation in humanized zebrafish model by moderating IL-6 and TNF-α cytokines. Journal of Inflammation Research. 2020;13:1219-1243.

  6. Balkrishna A, Solleti SK, Verma S, Varshney A. Application of humanized zebrafish model in the suppression of SARS-CoV-2 spike protein induced pathology by tri-herbal medicine coronil via cytokine modulation. Molecules. 2020;25(21):5091-5116.

  7. Balkrishna A, Solleti SK, Verma S, Varshney A. Validation of a novel zebrafish model of dengue virus (DENV-3) pathology using the pentaherbal medicine denguenil vati. Biomolecules. 2020;10(7):971-993.

  8. Balkrishna A, Rustagi Y, Bhattacharya K, Varshney A. Application of zebrafish model in the suppression of drug-induced cardiac hypertrophy by traditional Indian medicine yogendra ras. Biomolecules. 2020;10(4):600-621.

  9. Balasundaram A, David DC. Molecular modeling and docking analysis of aspirin with pde7b in the context of neuro-inflammation. Bioinformation. 2020;16(2):183-188.

Read how we drive change in your candidate development process

November 2024

Studying Protein Aggregates with Zebrafish

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Pentagrit Newsletter

March, 2026
​Vol. 2 No. 1
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Dr. Uppala Radhakrishnan on our latest issue, addressing three great questions in Lung Cancer Biomarkers.
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View Earlier Issues

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Pentagrit

66, VOC Street, IIT Park, Chennai,

India  - 600100

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